TAE Buffer 50X 500ML

TAE Buffer (50X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA) and as a running buffer for preparative work.Tris-Acetate-EDTA (TAE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation.Instructions for useTake a bottle of TAE Electrophoresis Buffer (50X) from specified storage conditions at 15-30°C and read the label.Ensure that the cap of the bottle is tight.Gently swirl the solution in the bottle to ensure homogeneity.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.Using aseptic/sterile technique under a laminar-flow culture hood and work according to established protocols.The TAE Electrophoresis Buffer Concentrate should be diluted to a working concentration of 1X before use.For each Electrophoresis, use fresh TAE Buffer at the working concentration of 1X